Posts Tagged ‘altered’

The interaction of path integration and terrestrial visual cues in navigating desert ants: what can we learn from path characteristics? [RESEARCH ARTICLE]

Cornelia Buehlmann, A. Sofia D. Fernandes, and Paul GrahamAnt foragers make use of multiple navigational cues to navigate through the world and the combination of innate navigational strategies and the learning of environmental information is the secr…

TRANSGENIC TOBACCO PLANTS FOR ENHANCED BIOETHANOL PRODUCTION

Genetically modified tobacco plants are provided having altered hexose accumulation. Methods are provided for producing ethanol from fermentation of tobacco biomass derived from the tobacco plants having altered hexose accumulation. The altered hexose …

Synthetic recording and in situ readout of lineage information in single cells

Reconstructing the lineage relationships and dynamic event histories of individual cells within their native spatial context is a long-standing challenge in biology. Many biological processes of interest occur in optically opaque or physically inaccessible contexts, necessitating approaches other than direct imaging. Here, we describe a new synthetic system that enables cells to record lineage information and event histories in the genome in a format that can be subsequently read out in single cells in situ. This system, termed Memory by Engineered Mutagenesis with Optical In situ Readout (MEMOIR), is based on a set of barcoded recording elements termed scratchpads. The state of a given scratchpad can be irreversibly altered by Cas9-based targeted mutagenesis, and read out in single cells through multiplexed single-molecule RNA fluorescence hybridization (smFISH). To demonstrate a proof of principle of MEMOIR, we engineered mouse embryonic stem (ES) cells to contain multiple scratchpads and other recording components. In these cells, scratchpads were altered in a progressive and stochastic fashion as cells proliferated. Analysis of the final states of scratchpads in single cells in situ enabled reconstruction of the lineage trees of cell colonies. Combining analysis of endogenous gene expression with lineage reconstruction in the same cells further allowed inference of the dynamic rates at which ES cells switch between two gene expression states. Finally, using simulations, we showed how parallel MEMOIR systems operating in the same cell can enable recording and readout of dynamic cellular event histories. MEMOIR thus provides a versatile platform for information recording and in situ, single cell readout across diverse biological systems.

Study links altered brain chemistry, behavioral impairments in fish exposed to elevated CO2

Study Links Altered Brain Chemistry, Behavioral Impairments in Fish Exposed to Elevated CO2 Research team studied damselfish behavior and physiology under ocean acidification conditions predicted for year 2300
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